Starch Hydrolysis by Amylase | Experiment

Starch Hydrolysis by Amylase | Experiment Polysaccharides are polymers of carbohydrates. They are made up from monosaccharides which are linked together by glycosidic bonds. Polysaccharides are divided into two main groups: structural polysaccharides and polysaccharides which are used as energy sources. Cellulose and chitin are classified as structural polysaccharides. Cellulose is major component of plant cell walls. It yields glucose when completely hydrolyzed. On the other hand Glycogen and starch are the polysaccharides which used as energy source; glycogen is found in animal cells and starch is found in plant cells. Plants use starch to use it to store glucose units for energy. It consists of two types of molecules: the linear and helical amylose and the branched amylopectin. Starch generally contains 20 to 25% amylose and 75 to 80% amylopectin. Amylose forms a colloidal dispersion in hot water whereas amylopectin is completely insoluble. 2.2) Hydrolysis of starch/amylase enzyme: Proteins which catalyze the chemical reactions are called enzymes. Biological catalyzes, enzymes, need specific conditions to be active since they are working in the cells. Temperature must be between 37 and 40 and ph must be neutral. An important metabolic enzyme is amylase that its function is to catalyze the hydrolysis of starch into glucose. Alpha-amylases are found in plants and in animals. Human saliva is rich in amylase, and the pancreas also secretes the enzyme. SUBSTRATE  Ã‚  Ã‚  Ã‚  Ã‚  ENZYME  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   PRODUCTS starch  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Amylase   ->  Ã‚   maltose + maltose + maltose starchs presence can be identified by using the iodine test. Starch and iodine gives blu-black color together and that helps to identify the presence of starch or iodine. The iodine molecule slips inside of the amylose coil. Iodine is not very soluble in water so the iodine reagent is made by dissolving iodine in water in the presence of potassium iodide. This makes a linear triiodide ion complex and this linear complex can easily slip into the coil of the starch. This forms the color. [3] starch + I2 à ¯Ã†â€™Ã‚   blue-black color 2.3) Spectrophotometry: A spectrophotometer is used to find the amount of radiant energy absorbed or transmitted by molecules in a solution as a function of wavelength. The wavelength which a certain molecule can absorb energy is different and therefore it can be used to determine the concentration of a specific type of solution. By comparing the amount of light that is absorbed by the sample with known concentration a calibration curve can be plotted and by using it concentration of the unknown sample can be determined. [5] 3) EQUIPMENT AND CHEMICALS 3.1) equipments: Beaker Laboratory scale Weighing dish Spatulas Tubes Tube rack Heater Water bath Plastic cuvettes Pipettes Dropper pH meter 3.2) chemicals: Distilled Water HCl NaCl KH2PO4 Na2HPO4 Starch solution Human salivary Iodine reagent 4) PROCEDURE 4.1) preparation of starch solution and enzyme solution: 20 g of potato starch was mixed with approximately 50 ml cold water. This mixture was then added to 900 ml of boiling water. Mixture was mixed well and it was cooled to room temperature and the total volume was raised to 1 ml by adding sufficient amount of water. Presence of the starch in the solution was tested by putting one drop from the mixture to glass plate and adding one drop of iodine reagent to it. Blue color means starch is present. Saliva sample was taken into tube and diluted with 9 ml water then, 60 ml of 0.5% NaCl was added. 4.2) Effect of the pH: 0.1 M KH2PO4 with pH 5, 6, 7 and Na2HPO4 with pH 8, 9 solutions were prepared as buffers, each buffer was prepared 20 ml only the buffer with 7 pH was prepared 100 ml. 5 test tubes were labeled and to each of them 5ml of the starch solution was put and to each test tube solution with different pH was added. 1 ml of the salivary enzyme solution was added to the tubes and it was mixed by shaking. it was waited for 10 minutes so that the hydrolysis reaction can proceed. 5 ml of HCl solution was added to 5 different tubes and from each of the previous tubes with the starch solution 0.5 ml was taken and added to tubes with HCl. 5 tubes were prepared again for 5 ml of iodine solution this time. From the tubes with HCl 0.5 ml was taken and added to the tubes with iodine. Absorbance values were measured by using spectrophotometer. 4.3) Effect of temperature: Water baths with 30  °C, 50  °C, 70  °C and 90  °C were prepared in beakers. Buffer solution with pH 7 was added to 5 tubes and 5 ml of starch solution was added to these tubes. All of these tubes were put in different water bath with different temperatures and they were waited in the bath until the temperatures reached the equilibrium. 1 ml of salivary enzyme was added to each tube. After 10 minutes the steps 4-7 in the effect of pH procedure were repeated. 5) DISCUSSION In this experiment our purpose was to hydrolyze starch with amylase enzyme and observe the effect of pH and temperature on this reaction. Before observing the pH and temperature effect first we prepared the solutions that we were going to use in the experiment. First we prepared the starch solution by mixing it first with cold water and then adding it into boiling water. We aimed to get the starch suspension form immediately and without any lumps by this procedure. In the first part of the experiment we observed the pH effect. To do that first we needed to prepare the buffer solutions with different ph values ranging between 5 and 9. We used two different solutions because of the buffering capacity of these two solutions. We made the arrangement of the pH by using HCl and NaOH. To decrease the pH we added HCl and to increase we added NaOH, we detected the pH by using pH meter. Each buffer with different pH values were mixed with starch solution and then salivary solution was added. W hich is the amylase enzyme and since its from the saliva it hydrolyzes amylose. After waiting for 10 minutes to reaction proceed we needed to stop the reaction, we did it by adding HCl. Then we added iodine solution to detect whether reaction took place or not since if enzyme functions starch in the solution will be hydrolyzed and this will lead to have light color of the solution; absorbance will be low. Since our body is in neutral pH we expect to have light colored solution at pH 7 and dark color at pH 5, 8 and 9. The same logic is valid for the temperature effect. The enzyme wont work in higher temperature values that can denature it like 90, 70 and maybe 50. Proteins absorbance values are expected to increase as the protein denaturizes. This can be explained by the surface of reflection of the light is increased. Denaturized form of protein has higher possibility to be interacted with the light from the spectrophotometer and thus absorbance will increase. Theoretically we would expect to have both absorbance vs. pH and absorbance vs. temperature graphs to have a min. point where we can say that is the point absorbance is in the min. point at that pH or temperature thus enzyme functions best at that point. In our graphs from the experiment we can observe these min values at approximately expected values. When we look at the temperature graph we see that absorbance is min at around 50 C. normally we would expect that proteins denaturize at that temperature, since human body is 37 °C min absorbance at 40 °C would be the correct result. This error might be because of that we didnt measured our test tubes temperatures after we put them in water bath, So maybe what we refer to as 50 °C in the data table is actually less than that value.

February Action †creative writing Essay

So, after my great holidays, back to reality. In this case, it meant moving flats. I moved out of my friends’ flat and in with two Mexican siblings, girl (spoilt acting student) and boy (pain in the ass lawyer student). (The statements in this part are highly influenced by later experiences with the two people, I got along with them and their family fine. ) And when I moved I found the flat under water. So the first thing I did was spend hours soaking towels. Apart from that the two were always nice to me, the guy was almost never there and the girl went home on weekends, so I had the place to myself a lot, which was great. The flat was on the ground floor, so despite great sunny weather outside, I lived in freezing conditions. But it was well-located for work and social life. My room consisted of a bed and a built in cupboard (already provided) and a very useful box (which I found on the street and stayed with me until I moved out). I started working too†¦ giving English classes in the Bank of Mexico, some 20 hours a week, all 1-1 classes with mostly enjoyable students. As I had already found a football team, it meant I had found friends who took me out, to the cinema and every sunday the PUMAS (one of the local teams) game was a must. I was introduced to the stadium, the anthem (!!!! ) and the during match entertainment (beer and crisps) and the after match entertainment (more beer, discussion of game, and strange activities like having electric shocks outside of the stadium). Although not completely my description of a great time, it was good fun nevertheless. I otherwise spent quite lazy weekends, enjoying the company of my friend Itziar and playing football. At the end of February, the football girls had a huge party, which was great great fun, a lovely, welcoming group of girls who know how to party. There I became better friends with a lot of them, which was very nice. The party was full of good food, dancing, dancing and dancing and when it ended, we decided to go to a friends’ house to continue the party, but we all ended up falling asleep there. So some good weekend action. I was invited by the family of my flatmates to Cuernavaca, which is the getaway for inhabitants of the big City. (btw, if New York City is the Big Apple, Mexico City must be something like the Big Papaya) I went out on the Saturday morning by bus and then spent quite a relaxed time in the house, which is quite a bit outside of the city center. We had a lovely meal and then they took me into the city center, where they, interestingly enough, put me on the tourist bus and I went around on that. Pity really, because the main square had some really interesting and fun dancing and singing going on, surrounded by people selling handcrafts and other oddities. So the bus ride was quite amusing, lots of historically interesting information that I forget while the guide is finishing the sentence. Cuernavaca is a nice city and the bus also took us on a walk through some park/ravine, which was also umm†¦ nice. Then in the evening I met the family to have supper at a very good Italian restaurant before we headed back to their house, where I fell right to sleep (tired from the party, obviously). Sunday just meant a long sleep, another nice meal and then the return journey back to the city. So I got a look at what Cuernavaca was like, which was enjoyable. Next time, I’ll just go by myself to see more of the place instead of seeing it wizzing by from the Touribus.

Appendicitis

In the position of the appendix, age of the patient, and degree of inflammation make the clinical presentation of appendicitis notoriously inconsistent. Statistics report that 1 of 5 cases of appendicitis is misdiagnosed; however, a normal appendix is found In 15-40% of patients who have an emergency appendectomy. Niwa et al reported an Interesting case of a young woman with recurrent pain In who was referred for appendicitis, treated with antibiotics, and was found to have an ppendiceal diverticulitis associated with a rare pelvic pseudocyst at laparotomy after 12 months. 15] Her condition was probably due to diverticular perforation of the pseudocyst_ Symptoms The classic history of anorexia and perlumblllcal pain followed by nausea, right lower quadrant (RLQ) pain, and vomiting occurs in only 50% of cases. Nausea is present in 61-92% of patients; anorexia is present in 74-78% of patients. Neither finding is statistically different from findings in patients who present to the emerg ency epartment with other etiologies of abdominal pain. In addition, when vomiting occurs, it nearly always follows the onset of pain.Vomiting that precedes pain Is suggestive of Intestinal obstruction, and the diagnosis of appendicitis should be reconsidered. Diarrhea or constipation Is noted In as many as of patients and should not be used to discard the possibility of appendicitis. The most common symptom of appendicitis is abdominal pain. Typically, symptoms begin as periumbilical or epigastric pain migrating to the right lower quadrant (RLQ) f the abdomen. This pain migration is the most discriminating feature of the patient's history, with a sensitivity and specificity of approximately 80%, a positive Ilkellhood ratio of 3. 8, and a negative likelihood ratio of 0. 5. [3] Patients usually Ile down, flex their hips, and draw their knees up to reduce movements and to avoid worsening their pain. Later, a worsening progressive pain along with vomiting, nausea, and anorexia are desc ribed by the patient. usually. a fever is not present at this stage. The duration of symptoms Is less than 48 hours In approximately 80% of adults but tends to be longer In elderly persons and In those with perforation.Approximately 2% of patients report duration of pain in excess of 2 weeks. A history of similar pain is reported in as many as 23% of cases, but this history of similar pain, in and of itself, should not be used to rule out the possibility of appendicitis. In addition to recording the history of the abdominal pain, obtain a complete summary of the recent personal history surrounding gastroenterologic, genitourinary, nd pneumologlc conditions, as well as consider gynecologic history In female patients.An inflamed appendix near the urinary bladder or ureter can cause Irrltatlve v0101ng symptoms ana nematurla or pyurla. cystltls In male patlents Is rare in the absence of instrumentation. Consider the possibility of an inflamed pelvic appendix in male patients with appare nt cystitis. Also consider the possibility of appendicitis in pediatric or adult patients who present with acute urinary retention.

Dialectical Tensions Is The Opposing Forces Of Which A...

Dialectical tensions are essentially the opposing forces of which a relationship works. Every relationship you are involved in should have tension and issues, it’s natural. I have many personal relationships where dialectical tensions are present. By studying interpersonal relationships and the different dialectical tensions, I have realized that I apply the different tensions on a daily basis without even knowing. By growing up with divorced parents, who do not particularly get along, I have had to compromise and really think about what I am going to say before I say it. Hearing low-blows about one parent made by the other parent really takes a toll on how you perceive the other person. I have lived a very blessed life and have wonderful parents, but in every relationship there are issues. My relationship with each parent allows me to use many strategies for handling dialectical tensions, but I specifically use a wider variety in my relationship with my father. An article by Donald K. Macrae states, â€Å"Divorce disrupts one s identity, economic and emotional dependency and disturbs primary relationships with family of origin, children, friends and colleagues† (Macrae1). I personally relate to this quote and it also directly relates to dialectical tensions. One of the main issues in my relationship with my dad is dependency. We struggle with autonomy versus connectedness, which is the dialectical tension resulting from the stimulus needed for independence and togethernessShow MoreRelatedThe Relational Dialectics Theory And The Genderlect Styles Theory1429 Words   |  6 Pagesthese forms of communication affect our relationships and vary between men and women. Numerous communication theories have been established regarding relationships and gender, but I will discuss two specific theories, the Relational Dialectics Theory and the Genderlect Styles Theory. 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